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Published on: Jul 29, 2021
2 minute read
By: Raj Dwivedi

What Tests Are Used To Manage Anemia And What Do They Mean?

The doctors use a number of tests to diagnose anemia. Hb (hemoglobin) is one of the key indicators used in diagnosis. A hemoglobin test is a blood test that measures how much hemoglobin you have in your red blood cells. A hemoglobin test is generally used for detecting:

In addition, a hemoglobin test can also be carried out to assess bone marrow's response to treatment, evaluate possible blood donation and check the levels of red blood cells in the body. There are certain conditions that may interfere with the results of the hemoglobin test. In such cases, it is recommended to get the test done again.

For the hemoglobin test, a sample of your blood is taken and tested in a clinical laboratory [read more about the process here]. The results are expressed as grams of hemoglobin per deciliter (g/dL) or micrograms per liter (mcg/L). Normal range for this test is 12g/dL - 18g/dL (12.1-18.0 g/L) for adults and 9g/dL - 13g/dL (9.0-13.3 g/L) for children. If the test result comes out to be less than 7 gm, it can indicate a serious condition known as severe anemia. In some cases, the doctors may order follow-up tests to assess the current condition of the patient.

Apart from the hemoglobin test, certain other blood tests can be used for detecting low RBCs (RBC count) or abnormal types of RBC (red cell indices) or hemoglobin (Hb). These include complete blood count tests, reticulocyte tests, iron tests, and red blood cell indices.

In some cases, the doctor may opt to do a bone marrow aspiration and exam to check for any abnormalities in the bone marrow cells. In such cases, it of red blood cells is karyotype to rule out chromosomal disorders.

The most common reason for having an inadequate number of red blood cells in the body is an iron deficiency or anemia. Iron plays a significant role in the production of hemoglobin, which is responsible for carrying oxygen from the lungs to the tissues of other parts of the body. Hence, people suffering from iron are known as sickle cell trait. The tests include:

Complete blood count test - The complete blood cell count (CBC) is a routine test that checks the different components of your blood like number of red blood cells, white blood cells and platelets. It also has information about the size and hemoglobin content in each of these cells. A CBC includes tests for the following:

Reticulocyte test - This is a blood test that measures the reticulocytes (immature red blood cells) in the bloodstream. A high level of reticulocytes can indicate increased production of red blood cells by the bone marrow to compensate for anemia, or destruction of very early stage RBCs which are still unable to mature.

Iron tests - Iron is an essential component for RBC production. A test to assess the iron level in your blood is called a serum ferritin test, which measures the amount of iron stored in the body's cells and tissues. Another way to evaluate low iron levels is by carrying out a transferrin saturation test. In this case, the lab technician will extract serum from your blood sample and measure the amount of transferrin protein present in it. Transferrin is a protein that carries iron to the bone marrow where red blood cells are produced. Thus, if there is an insufficient amount of transferrin protein present in the serum, it shows that there is an inadequate level of stored iron.

Hemoglobin tests - A hemoglobin test is generally carried out to detect anemia, especially if the result of CBC tests are inconclusive. During this test, a small sample of blood is collected from your vein and sent to the laboratory for analysis. The test measures the amount of hemoglobin in your red blood cells.

Red cell indices - Red cell indices are tests that measure the size and hemoglobin content of the red blood cells. The values obtained from these tests can be used to detect anemia and other blood disorders.

Karyotype test - It is a lab test used to evaluate chromosomal abnormalities in red blood cells or bone marrow cells

Low blood cell counts also have other causes, such as infections or tumors. In some cases, low blood cells may indicate a more serious condition like bone marrow failure which requires immediate medical intervention to save the patient from death.

Thus, if you are suffering from any of these conditions and symptoms and suspect that you may be anemic, check with your doctor for the necessary tests to confirm or rule out your suspicions. Early detection and treatment of anemia can save you from serious complications like heart attacks, strokes, blood clots, etc.

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Sleep Debt and Its Impact on the Body: Hidden Risks of Chronic Sleep Loss

Sleep is not a luxury; it is a biological necessity. Yet in today’s fast-paced world, many people consistently sacrifice sleep due to work demands, screen time and stress. Over time, insufficient sleep accumulates into what is known as sleep debt. Understanding sleep debt and its impact on the body is essential because chronic sleep loss silently affects nearly every organ system.

According to the World Health Organization and global sleep research, chronic sleep deprivation is associated with increased risk of cardiovascular disease, metabolic disorders and mental health conditions.

What Is Sleep Debt?

Sleep debt refers to the cumulative effect of not getting enough sleep.

For example:

if you need 8 hours but sleep 6 hours daily
you accumulate 2 hours of sleep debt per night

Over a week, that equals 14 hours of lost sleep.

This deficit places strain on the body.

Why Sleep Is Critical for Health

Sleep supports:

brain function
immune response
hormonal regulation
tissue repair
memory consolidation

Without adequate sleep, these processes become impaired.

Immediate Effects of Sleep Debt

Short-term consequences include:

daytime fatigue
poor concentration
mood swings
irritability
reduced reaction time

Even one night of poor sleep affects cognitive performance.

Sleep Debt and Hormonal Imbalance

Sleep regulates several key hormones.

Chronic sleep loss disrupts:

cortisol
insulin
leptin and ghrelin (hunger hormones)

This imbalance affects appetite, stress and metabolism.

Impact on Metabolic Health

Sleep debt increases:

insulin resistance
blood sugar fluctuations
abdominal fat accumulation

ICMR and NFHS-5 data show rising metabolic disorders in India, partly linked to lifestyle patterns including poor sleep.

Increased Risk of Obesity

When sleep is insufficient:

appetite hormones increase
cravings for high-calorie foods rise
impulse control weakens

Sleep deprivation promotes weight gain.

Sleep Debt and Immunity

The immune system relies heavily on sleep.

Chronic sleep loss:

reduces infection-fighting cells
increases inflammation
slows recovery from illness

Lancet research confirms that sleep deprivation weakens immune response.

Cardiovascular Consequences

Sleep debt increases risk of:

hypertension
heart disease
stroke

Poor sleep affects blood pressure regulation and vascular health.

Mental Health Impact

Sleep and mental health are deeply connected.

Sleep debt contributes to:

anxiety
depression
emotional instability

Chronic insomnia is both a cause and consequence of mental health disorders.

Cognitive Decline and Brain Health

Lack of sleep impairs:

memory
attention
decision-making

Over time, chronic sleep deprivation may increase risk of neurodegenerative disorders.

Sleep Debt and Inflammation

Chronic sleep loss elevates inflammatory markers.

Persistent inflammation contributes to:

metabolic syndrome
cardiovascular disease
autoimmune conditions

Preventing sleep debt reduces systemic inflammation.

Can Sleep Debt Be Recovered?

Short-term sleep debt can be partially recovered through:

consistent longer sleep
improved sleep hygiene

However, chronic sleep deprivation requires long-term behavioural changes.

Weekend “catch-up sleep” offers temporary relief but does not fully reverse long-standing sleep debt.

Warning Signs of Sleep Debt

Common indicators include:

reliance on caffeine
difficulty waking up
daytime drowsiness
poor focus
frequent illness

Persistent symptoms require lifestyle correction.

Practical Strategies to Reduce Sleep Debt

Maintain a Consistent Sleep Schedule

Go to bed and wake up at the same time daily.

Limit Screen Exposure Before Bed

Blue light suppresses melatonin production.

Create a Sleep-Friendly Environment

Dark, quiet and cool environments improve sleep quality.

Manage Stress

Relaxation techniques reduce cortisol levels and support sleep.

Avoid Heavy Meals and Caffeine at Night

These disrupt sleep cycles.

Role of Preventive Health Checkups

Screening helps detect:

hypertension
metabolic imbalance
stress-related conditions

Sleep quality assessment should be part of preventive care.

Long-Term Benefits of Adequate Sleep

Restorative sleep supports:

stable mood
strong immunity
healthy weight
improved productivity
reduced disease risk

Sleep is foundational to wellness.

Conclusion

Understanding sleep debt and its impact on the body highlights the critical role sleep plays in maintaining physical and mental health. Chronic sleep loss disrupts hormones, weakens immunity, increases metabolic risk and affects heart health. While occasional sleep loss may be manageable, consistent deprivation carries serious long-term consequences. Prioritising adequate, high-quality sleep is one of the most powerful steps toward protecting overall health and preventing chronic disease.

References

World Health Organization (WHO) – Sleep and Non-Communicable Diseases
Indian Council of Medical Research (ICMR) – Lifestyle Disorders and Sleep Patterns
National Family Health Survey (NFHS-5) – Adult Health Indicators
Lancet – Sleep Deprivation and Chronic Disease Research
NITI Aayog – Preventive Healthcare and Lifestyle Risk Factors

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